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. Author manuscript; available in PMC: 2016 Sep 18.
Published in final edited form as: ACS Chem Biol. 2015 Jun 23;10(9):2057–2064. doi: 10.1021/acschembio.5b00197

Figure 6. Probe labeling profile of wild-type HEK293 cells.

Figure 6

(a) Protein blot analysis performed on cellular fractions from wt HEK293 cells incubated with 1 (left membrane) or 2 (right membrane). The total cell lysate of NAAA-HEK293 cells incubated with 1 was used as positive control (lane 5, left membrane). (b) Protein blot analysis of 1 incubated samples enriched on streptavidin-agarose and eluted. Blotting membranes were probed with a streptavidin-HRP conjugate. C: cytosol; M: membranes; T: total lysate