Table 4.
Humoral and cellular responses to vaccination in the eleven evaluable FL patients on study.
| Patient UPIN1 | Tumor class | Number of vaccinations2 | Immune status (lymphocyte number) | Humoral response3 | Cellular response8 | ||||
|---|---|---|---|---|---|---|---|---|---|
| ELISA | Flow Cytometry7 | T-cell proliferation | ELISpot | ||||||
| KLH4 | Plant Id5 | Hybridoma Id6 | |||||||
| U001 | IgM-lambda | 12 of 12 | Normal total lymphocyte count Low CD4+ T-cell count |
+ | + | + | + | + | − |
|
| |||||||||
| T003 | IgM-kappa | 12 of 12 | Lymphopenic (all subsets) | + | − | − | ND | − | − |
|
| |||||||||
| T006 | IgG-lambda | 12 of 12 | Normal total lymphocyte count Low CD4+ T-cell count |
+ | − | ND | ND | + | + |
|
| |||||||||
| T008 | IgM-kappa | 7 of 12 | Lymphopenic Normal CD8+ T-cell count |
+ | − | − | ND | − | − |
|
| |||||||||
| T010 | IgG-kappa | 9 of 12 | Normal total lymphocyte count Low CD4+ T-cell count |
− | − | − | ND | + | + |
|
| |||||||||
| U011 | IgM-kappa | 12 of 12 | Lymphopenic (all T-cell subsets) | + | + | + | + | + | + |
|
| |||||||||
| U016 | IgM-lambda | 12 of 12 | Lymphopenic (all T-cell subsets) | + | − | − | ND | + | − |
|
| |||||||||
| U017 | IgM-kappa IgG-kappa |
12 of 12 | Normal total lymphocyte count Low CD4+ T-cell count |
+ | − | ND | ND | + | + |
|
| |||||||||
| T021 | IgG-lambda | 9 of 11 | Normal total lymphocyte count Low CD4+ T-cell count Borderline CD19+ B-cell count |
− | + | − | − | + | − |
|
| |||||||||
| T022 | IgG-kappa | 12 of 12 | Normal total lymphocyte count Low CD4+ T-cell count Low CD19+ B-cell count |
− | − | − | ND | + | − |
|
| |||||||||
| U026 | IgM-lambda | 12 of 12 | Lymphopenic (all subsets) | + | − | ND | ND | + | − |
+ = Positive response; − = Negative response; ND = Not determined. 1 = Unique Patient Identification Number. 2 = Number of vaccines administered of the maximum 12 vaccines scheduled. 3 = Humoral responses were measured by ELISA and by flow cytometry. 4 = Specific response to KLH by ELISA was included to assess patients' overall immune responsiveness. 5 = Plant-made idiotype administered in the clinical study. 6 = Equivalent Id made in hybridoma to cross-check immune response to plant-made Id. 7 = Flow cytometry was used only as a confirmatory assay for humoral response when positive ELISA responses were measured. 8 = Cellular immune responses were measured by T-cell proliferation and by ELISpot.