Table 1. Applied Primers Sequences a.
| Name/Primer | Sequence (5’ - 3’) | Annealing Temperature, °C | Size of Ampelicon, bp | Use (s) |
|---|---|---|---|---|
| hexaaplex PCR (h- PCR) | 52 | Detection of carbapenemase in the molecular classes A and B | ||
| IMP-1F | CTATCCCCACGTATGCATCTGA | 174 | ||
| IMP-1R | TTTCAGGCAACCAAACCACTA | |||
| VIM-2F | TGGTCTATTTGACCGCGTCTAT | 210 | ||
| VIM-2R | CATCACCATCACGGACAATGAG | |||
| NDM-1F | ACTGGATCAAGCAGGAGATCAA | 279 | ||
| NDM-1R | CGGTGATATTGTCACTGGTGTG | |||
| KPCF | ATATCTGACAACAGGCATGACG | 315 | ||
| KPCR | GTCGTGTTTCCCTTTAGCCAAT | |||
| bla GESF | TCGAAAACTTTCATATGGGCCG | 411 | ||
| bla GESR | TCTTTAGGAAAACCCGCTCGTA | |||
| SPM-1F | ATTGATTCGGACGTTTTCGTCG | 540 | ||
| SPM-1R | CAGATAACCAAGTTCCTTCGGC | |||
| ISAba1F/OXA-51 R | 51 | 227 | ISAba1detection upstream of bla OXA-51 -like genes | |
| ISAba1F | AAGCATGATGAGCGCAAAG | |||
| OXA-51 R | GGTGAGCAGGCTGAAATAAAA | |||
| ISAba1F/OXA-23 R | 44 | 321 | ISAba1 detection upstream of bla OXA-23 -like genes | |
| ISAba1F | TGAGATGTGTCATAGTATTC | |||
| OXA-23 R | AGAGCATTACCATATAGATT | |||
| ISAba4F/OXA-23 R | 41 | 327 | ISAba4detection upstream of bla OXA-51 -like genes | |
| ISAba4F | CACAATTTCTGATAAAGATA | |||
| OXA-23 R | TTTATTAAATTATGCTGAAC |
a All References are belong to this study.