Fig. 3.

Overexpression of miRNA128 reduces Dcx protein levels in N2A cells. a–c The samples analyzed were mock-treated Neuro2A cells (control transfection without miRNA-128-RFP expressing plasmid) or Neuro2A cells treated with miRNA-128-RFP expressing plasmid. a Taqman quantitative RT-PCR of the mature miRNA-128 transcripts normalized against the housekeeping small non-coding RNA snoRNA55. Transcript levels are indicated as fold change respect to the control (mock) that is set to 1. n = 3, bars represent the mean ± standard error of the mean. b Western blot showing the Dcx and Gapdh (loading control) protein levels in mock and miRNA-128 over-expressing cells. c Quantitative RT-PCR of the indicated transcripts. Transcript levels are indicated as fold change respect to the control (mock) that is set to 1. Transcripts were normalized against the housekeeping gene Srp14. n = 4, bars represent the mean ± standard error of the mean. Two-tailed t test was applied for statistical analysis. NS not significant; p = 0.4