Fig. 4.

Overexpression of miRNA128 reduces Dcx protein levels in “in vitro” differentiating adult neural stem cells. a, b Adult neural progenitor cells were transfected with miRNA-Ctr-RFP (control) or mirRNA-128-RFP (experiment) expressing plasmids and induced to differentiate by growth factor withdrawal. Analysis was done 6 days later. a Representative pictures of the immunostaining of Dcx, GFAP and RFP on control and experiment cells. DAPI visualizes the DNA. Transfected cells are RFP positive. Arrows indicate Dcx/RFP positive cells; arrowheads indicate GFAP/RFP positive cells. b Quantification of Dcx and GFAP among the transfected cells. Bars show the average ± the standard error of the mean (n = 3). The percentage of marker-positive cells among the total RFP-positive population was calculated on three biological replicates. At least 100 cells per experimental group were analyzed. Two-tailed t test was applied for statistical analysis. Asterisks indicate statistically relevant differences; p < 0.05