Fig. 5.

RNAi components are associated with chromatin in differentiating adult neural stem cells. a Scheme of the procedure followed to fractionate neural progenitor cells. Insoluble fractions (pellets) were dissolved in RIPA buffer (see “Methods”). Chromatin associated proteins should be found in fractions P1 and S2. b, c Equivalent amounts of the resulting protein samples from undifferentiated or differentiating (3 days) adult neural progenitor cells were analyzed by western blot for the presence of the indicated proteins. Gapdh (glyceraldehyde 3-phosphate dehydrogenase) serves as a chromatin unbound marker; Pol II (RNA Polymerase II) is a chromatin bound marker. β-cat β-catenin, Ago2 Argonaute-2. Three independent biological samples have been analyzed. Shown are representative pictures