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Analytical and semipreparative HPLC conditions for HBS α-helicesa.
| Analytical scale (flow rate 1 ml min−1) |
Semipreparative scale (flow rate 5 ml min−1) |
||
|---|---|---|---|
| Time (min) | Solvent B (% (vol/vol) in solvent A) | Time (min) | Solvent B (% (vol/vol) in solvent A) |
| 0 | 5 | 0 | 5 |
| 12 | 95 | 45 | 95 |
| 15 | 100 | 50 | 100 |
| 17 | 5 | 55 | 5 |
Solvent A: 0.1% (vol/vol) TFA in H20; solvent B: 0.1% (vol/vol) TFA in acetonitrile.
