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. Author manuscript; available in PMC: 2015 Sep 20.
Published in final edited form as: Am J Med Genet B Neuropsychiatr Genet. 2010 Oct 18;0(8):1434–1447. doi: 10.1002/ajmg.b.31125

Figure 5.

Figure 5

Enhancement of E3b inclusion maps to SNPs flanking E3b. Top panel: Diagram of mapping strategy by exchange of DNA between putative “PITT risk” and “non-risk” constructs. The location of primers for RT-PCR is shown by the black arrows. Bottom panel: Results of RT-PCR assay after transfection into SH-SY5Y cells. Procedures as in Figure 4. Exchanging the region immediately flanking E3b confers increased inclusion of E3b on a “non-risk” construct, as shown by quantification of the proportion of E3b(+) to total DAT mRNA at the right. Student’s t-test showed that the difference in E3b inclusion between the swap construct and the non-risk construct was significant (t = 5.00, P < 0.001), whereas the swap construct and the risk construct did not differ significantly (t = 0.218, P > 0.1).