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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Neurovirol. 2015 Mar 20;21(6):679–687. doi: 10.1007/s13365-015-0326-2

Figure 2. Effect of coexpression of p300 and p65 Lys to Arg mutants on transactivation of the JCV early promoter.

Figure 2

TC620 cells were transfected with the reporter plasmid JCVE-LUC and NF-KB p65 expression plasmid (wild-type, K218R, K221R or K310R) together with expression plasmid for p300 (0, 0.25 and 0.5 μg), harvested and assayed for luciferase activity. Activities were normalized to untreated controls. The bar represents one standard deviation. The histogram at the bottom shows quantification of the Western. The experiment was performed at least twice.