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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Metab Eng. 2015 Jul 10;31:74–83. doi: 10.1016/j.ymben.2015.06.010

Figure 4.

Figure 4

De novo production of norcoclaurine in engineered yeast. (A) LC-MS/MS MRM analysis of norcoclaurine production in CSY1051 with RnTyrH (pCS3231) and CjNCS (pCS3241). Norcoclaurine standard (2.5 nM) is shown in black. (B) Norcoclaurine production as a function of NCS species variant. CSY1051 strains harboring plasmids with RnTyrH (pCS3231) and either TfNCS (pCS3240), PsNCS (pCS3242), or CjNCS (pCS3241) were grown in selective media lacking tyrosine and supplemented with 2 mM ascorbic acid for 96 hours before analysis. (C) Norcoclaurine production as a function of TyrH mutant. CSY1051 strains harboring plasmids with RnTyrH mutants (pCS3231-pCS3239) and CjNCS (pCS3241) were grown in selective media lacking tyrosine and supplemented with 2 mM ascorbic acid for 96 hours before analysis. Data is reported as the mean ± s.d. of at least 3 independent experiments.