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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Immunol. 2015 Aug 28;195(7):3119–3128. doi: 10.4049/jimmunol.1402670

Figure 3. Memory phenotype in the absence of ADAP occurs after CD8 T cells exit the thymus.

Figure 3

Cells from thymus (A) or spleen (B–D) were obtained from WT and ADAP−/− unimmunized 8–12 week old adult mice and the expression of surface molecules was analyzed on CD8+ CD4− TCR-β+ cells. (A) Absolute number of total CD8SP TCR-βhi, CD44lo CD122lo, and CD44hi CD122hi cells from the thymus. (B) CD24 and QA2 staining from WT and ADAP−/− spleens. Numbers represent the percentage of RTE, 1–2 week post egress and mature CD8 T cells. Recent thymic emigrants (RTEs) are defined as CD24hi Qa2lo. Cells that have emigrated between 1–2 weeks prior are defined as CD24hi Qa2hi. Mature cells are defined as CD24lo Qa2hi. (C) Absolute number of RTE, 1–2 week post egress and mature CD8 T cells. (D) The percentage of MP from RTE, 1–2 week post egress and mature CD8 T cell populations. The results (A) are compiled from at least five independent experiments (± SEM) with at least two mice per experiment, and (C and D) are compiled from at least three independent experiments with at least three mice per experiment.