Figure 8.

Oligoclonal ß-cell autoreactive CD8 and CD4 T-cells can induce T1D in NSG mice.

Total splenocytes from recently diabetic NOD-NY8.3 females were adjusted to inject i.v. 2.5×10⁵ NY8.3 CD8 T-cells into 8-10 week old NOD-scid or NSG females. A) Diabetes incidence study showing representative results from 3 experiments. PBL from mice receiving NY8.3 T-cells were analyzed by FACS at 3 weeks post transfer, for presence of B) CD4⁺, CD8⁺, tetramer specific NY8.3 CD8 T-cells and C) for presence of CD4⁺Foxp3⁺ T-cells. A total of 1.5×10⁶ purified splenic CD4⁺ T-cells from NOD-BDC2.5 mice were injected i.v. into NOD-scid or NSG mice. D) Mice were monitored for diabetes development. E) At two weeks post transfer, the proportion of circulating Foxp3⁺ Tregs was assessed by FACS in PBL isolated from mice receiving CD4⁺ T-cells from NOD-BDC2.5 donors. F) A total of 2×10⁵ purified CD4⁺CD25⁻ T-cell from NOD-BDC2.5 donors were injected i.v. into NOD-scid (n = 10) or NSG (n = 10) mice, diabetes incidence is shown. Data represent mean ± SEM, ** p<0.001, **** p<0.00001, non parametric T-test (Mann-Whitney).