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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Immunol. 2015 Aug 31;195(7):3071–3085. doi: 10.4049/jimmunol.1500153

Figure 2. Microbiota-mediated increase in splenic MZ B and CD11b+ cells in Il10−/− mice.

Figure 2

(A) The percentage of MZ B cells (gated on B220) (left panel), CD11b+ myeloid cells (middle panel) and Fo B cells (gated on B220) (right panel) was quantitated in Il10−/− Jax housed alone or cohoused with Il10−/− mice from our colony for four weeks. Each data point represents a single mouse. (B) WT and Il10−/− Jax mice were cohoused with Il10−/− mice from our colony and the percentage of MZ B cells (gated on B220) and CD11b+ myeloid cells in the spleen was determined on day 7, 21, 28 and 35. Data are shown as the mean ± SE (n = 3). (C) The percentage (top panels) and absolute number (bottoms panels) of splenic MZ B cells (gated on B220) (left panels) and CD11b+ myeloid cells (right panels) in WT Jax cohoused and Il10−/− Jax and Il10−/− Jax cohoused mice (four weeks) with and without antibiotic treatment was determined by flow cytometry. Data are shown as the mean ± SE of two experiments (n = 6–7). *p<0.05, **p<0.01.