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. Author manuscript; available in PMC: 2016 Sep 17.
Published in final edited form as: Mol Cell. 2015 Sep 10;59(6):1011–1024. doi: 10.1016/j.molcel.2015.07.029

Fig. 5. Regulation and function of Chk1 during recovery.

Fig. 5

A. Levels of pDNA-PK, pATM, pChk1 and CDC25A were analyzed in ATRi-treated U2OS cells at the indicated times. B. U2OS cells were treated with ATRi, ATMi, DNA-PKi, or the combinations of these inhibitors. Levels of pDNA-PK, pATM, pChk1 and CDC25A were analyzed 8 h after treatment. C. The percentage of replication tracts containing fired origins was determined in RPE1 cells treated with DMSO or ATRi at the indicated times. Error bars: S.D. (n=3 experiments). **P<0.01; ***P<0.001. D. RPE1 cells were treated with DMSO or various inhibitors as indicated. The inter-origin distance was analyzed using DNA fiber assay at the indicated times. Error bars: S.E.M. (n=25 to 67 as indicated). ****, P<0.0001; n.s., not significant. E-F. RPE1 cells were treated with DMSO or various inhibitors as indicated. The length of continuous replication tracts was determined using DNA fiber assay at the indicated times (>600 forks per condition, n=3 experiments). See also Fig. S5.