Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Protein Expr Purif. 2015 Aug 6;115:1–10. doi: 10.1016/j.pep.2015.08.005

Figure 1.

Figure 1

Comparison of 1D-HNCO slices which illustrate differences in carbon-13 isotope incorporation (100% 15N in all samples) as a result of implementing (left column) exact 10%w/w or 20%w/w fractional carbon-13 enrichment versus (right column) enhanced fractional enrichment, both using 200 mg u-13C-glucose (10%w/w). These data are also summarized in Table 1. (a) The 1D-HNCO slices (256 transients each) indicate that opt-10%w/w-BDF samples produce SNR similar to the exact 20%w/w sample. An independent trial (b) (128 transients each) contrasts an opt-10%w/w-BDF sample with an exact 10%w/w sample, where the almost two fold improvement is observed.