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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Protein Expr Purif. 2015 Aug 6;115:1–10. doi: 10.1016/j.pep.2015.08.005

Figure 7.

Figure 7

Representative strips comparing the same opt-10%w/w-BDF HNCACB experiment in Figure 6 to an exact 10%w/w-BDF sample demonstrate that the opt-BDF protocol disrupts the CACB connectivity in ILV residues of ubiquitin, but 10% enrichment at the beginning of growth does not. The 10%-BDF sample was prepared to about 1 mM and the HNCACB acquired for it consumed 22 hrs (8 scans per increment for a 64[13C] x 32[15N] matrix, sw13C=80 ppm, sw15N=35 ppm, 1.2 second recycle time, 150 ms acquisition).