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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Protein Expr Purif. 2015 Aug 6;115:1–10. doi: 10.1016/j.pep.2015.08.005

Figure 8.

Figure 8

Adjacent 13C-13C spin labels in the backbone of proteins prepared by opt-10%w/w-BDF methods are demonstrated by the projection of all planes of a 3D-HN(CA)CO acquired on a 0.40 mM opt-10%w/w-BDF sample non-uniformly acquired (32 scans per increment nonuniformly spanning a 64[13C] x 40[15N] matrix with 500 samples, sw13C=20 ppm, sw15N=35 ppm, 2.0 second recycle time, 150 ms acquisition) in 38 hrs. This and all other experiments were performed on a 600 MHz spectrometer using a room-temperature probe.