. Author manuscript; available in PMC: 2016 Nov 1.

Published in final edited form as: Protein Expr Purif. 2015 Aug 6;115:1–10. doi: 10.1016/j.pep.2015.08.005

Table 1.

Enhanced fractional enrichment of carbon-13 incorporation into yeast ubiquitin as measured by comparing the 1D-HNCO integral values (range: 9.5 to 7.0 ppm) via and optimized BDF methods. Each trial is a pair of the control (exact 10%^w/w-BDF) and enhance (opt-10%^w/w-BDF samples, and is independent of all other trials. Thus this table summarizes data from 12 distinct samples.

Trial	Glucose (g) (u-¹³C/na)	Induction Temp	Induction OD₆₀₀ (ctrl/opt)	Induction Duration	Incorporation^#
1	0.20/1.80	18	0.8/0.8	64 hr	18%	Fig1a
2		18	0.8/0.8	64 hr	18%	Fig1b
3		18	0.74/0.74	48 hr	17%	Fig2a
4		37	0.79/0.74	5.5 hr	20%	Fig2b
5	0.40/3.60	18	1.00/0.99	60 hr	17%	Fig3a
6		18	1.10/1.10	64 hr	18%	Fig3b

Estimated error 1%.

Number of transient recorded for the 1D-HNCO experiments are 1: 256, 2: 128, 3: 512, 4: 512, 5: 512, 6, 4096.