. Author manuscript; available in PMC: 2016 Nov 1.

Published in final edited form as: Protein Expr Purif. 2015 Aug 6;115:1–10. doi: 10.1016/j.pep.2015.08.005

Table 2.

Several conditions bearing the common theme that induction occurred on or after the onset of stationary phase all result in a much lower enhancement of carbon-13 incorporation. Contrasting the results in Tables 1 and 2 supports that enhanced carbon-13 incorporation is higher when the fractional quantity of u-¹³C-glucose is added near the end of log phase growth, prior to stationary phase. Number of transient recorded for the 1D-HNCO experiments are 7: 512, 8: 384, 9, 64.

Trial	Glucose (g) (u-¹³C/na)	Induction Temp	Induction OD₆₀₀ (ctrl/opt)	Induction Duration	Incorporation^#
7	0.40/3.60	18	1.00/1.00	60 hr	13%	Fig4a
8	0.20/1.80	18	0.81/0.81	48 hr	12%	Fig4b
9	0.40/1.60	18	0.87/0.83	64 hr	23%	Fig5