Figure 5.

Effects of IL1A on Expression of Endogenous MYOC and SELE (A) western blot of lysates from cultures of TM-1 cells stably transduced with empty lentivirus vector. Cultures were treated for 72 h with IL1A (10 ng/ml), IL1RA (100 ng/ml), or both IL1A and IL1RA. Blots were probed for MYOC and SELE. ACTB was used as a control to demonstrate equal loading of gel lanes. B: Western blot of lysates from two primary trabecular meshwork cell lines derived from separate individuals (primary line 1 and primary line 2). Cells were plated and cultures were allowed to grow to confluence. They were then treated with IL1A (10 ng/mL) or dexamethasone (100 nM) for 10 days.