Table 1. Primers used in qPCR studies.
Genes | Accession number | Forward (5′-3′) | Reverse (5′-3′) | Annealing Temperature(°C) | Product size (base pairs) | |
---|---|---|---|---|---|---|
CK4 |
XM_008256495.1 |
CAACCTGAAGACCACCAAGA |
CAGAGTCTGGCACTGCTTT |
60 |
100 |
|
MUC5AC |
XM_008253634.1 |
TGATGACCAACCAGGTCATTT |
GGGATGGTCACGTACATCTTG |
60 |
106 |
|
GAPDH | NM_001082253 | GGTCGGAGTGAACGGATTT | TGTAGTGGAGGTCAATGAATGG | 60 | 113 |