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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Arch Biochem Biophys. 2015 Aug 14;583:96–104. doi: 10.1016/j.abb.2015.08.005

Figure 3.

Figure 3

A, C) Deconvoluted ESI-MS spectra of the reaction mixture obtained from 1 mM EdAG and 50 μM Grx-1 or Grx-2 at 8 and 10 hrs, respectively. B, D) Time course of the EdAG adduct formation with Grx-1 and Grx-2, respectively. The reactions were conducted at 37 °C in PBS, pH 7.4, and in presence of 250 μM TCEP. The percentage of adducted protein was calculated from the fractional area of the most intense charge state (i.e. [M+11H]+11 for Grx-1/EdAG-Grx-1 and [M+16H]+16 for Grx-2/EdAG-Grx-2) in the MS spectrum.