Figure 6.

Catalytic activity of Grx’s after reaction with EdAG. Samples of 50 μM Grx’s in PBS pH 7.4 were incubated at room temperature (Grx-1) or at 4 °C (Grx-2) in presence or in the absence of 1 mM EdAG. After 24 hours, HED activity assays were conducted as described in the material and methods section. With partial (~35%) adduction by EdAG, Grx catalytic activity is decreased ~47%. The loss of activity above the level of adduction is likely due to oxidation and denaturation.