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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Int J Obes (Lond). 2015 Mar 12;39(6):967–976. doi: 10.1038/ijo.2015.23

Figure 2.

Figure 2

Resveratrol promoted formation of brown-like adipocytes in the differentiated iWAT SVC cells from wild-type mice after 7-day adipogenic differentiation. A) Relative mRNA levels of brown adipocytes selective genes (PRDM16, UCP1, Cidea, Elovl3 and PGC-1a) and beige adipocytes selective genes (CD137, Tbx1 and TMEM26). B) UCP1 immunofluorescence staining for UCP1 in the differentiated iWAT SVC. Nuclei were stained with DAPI (scale bar, 100 μm). C, D) Western blot analysis of brown adipocytes selective genes (UCP1, PRDM16, Cyto C, and PDH) in the differentiated iWAT SVC, and β-tubulin was used as loading control (C); Mean ± SEM of immunoblotting bands of UCP1, PRDM16, Cyto C and PDH (D). The intensities of the bands were expressed as arbitrary units. E) Basal O2 consumption of differentiated iWAT SVC from control and resveratrol treated groups. *P < 0.05, **P < 0.01 and *** P < 0.001 versus control.