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. 2015 Sep 10;9(5):451–458. doi: 10.4162/nrp.2015.9.5.451

Fig. 3. Effect of EPO and cytokines on colony-forming cells.

Fig. 3

Bone marrow cells derived from Balb/c mice were cultured in RPMI1640 medium with 10% FCS (base media). For colony formation, IL-3, SCF, and EPO were added to the media (completed media), and evaluated. Colony formation was photographed by microscope (A). After 14 days of incubation, the colony-forming unit-granulocyte/erythrocyte/macrophage/megakaryocyte (CFU-GEMM) and erythroid (BFU-E) were determined (B). Total colony: (GFU-GEMM+BFU-E). The data were expressed as the mean±SEM of three separate experiments. BFU-E had multicentric (burst) erythroid colonies with red or pink color. IL-3: interleukin-3; SCF: stem cell factor; EPO: erythropoietin; GFU-GEMM: colony-forming unit-granulocyte/erythrocyte/macrophage/megakaryocyte; BFU-E: burstforming unit erythroid.