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. 2015 Sep 10;9(5):451–458. doi: 10.4162/nrp.2015.9.5.451

Fig. 4. Effect of NFA and FAB on colony formation in splenocyte conditioned media containing EPO.

Fig. 4

Bone marrow cells derived from Balb/c mice were cultured in RPMI1640 medium with 10% FCS (base media). Colony formation of bone marrow cells was induced with SCM and EPO. PHA, NFA, and FAB were added to stimulate colony formation, and evaluated (A). The numbers of total colonies and BFU-E were determined in NFA or FAB-treated SCMs containing different concentrations (B and C).Data from four separate experiments are expressed as the mean ± SEM. Means with different superscript letters indicated significant differences at P < 0.05 by Duncan's multiple range tests. NFA: non-fermented antler extract;. FAB: antler extract fermented by B. subtilis ; EPO: erythropoietin; SCM: splenocyte conditioned media; BFU-E: burstforming unit erythroid. PHA was positive control and its concentration was 10 µg/mL.