Table 1.

Antibodies, dilution, antigen retrieval method, and incubation time of different biomarkers.

Marker	Antibody	Dilution	Antigen retrieval method	Incubation time
EGFR	Mouse monoclonal antibody, clone 111.6, Lab Vision	1 : 400	Proteinase XXV (1 mg/mL PBS), 15 min at 37°C	2 h, RT
P53	Rabbit monoclonal antibody, clone Y5, Lab Vision	1 : 100	10 mM citrate buffer pH 6.0, pressure cooker	30 min, RT
Ki-67	Rabbit monoclonal antibody, clone SP6, Lab Vision	1 : 200	10 mM citrate buffer pH 6.0, pressure cooker	30 min, RT