Figure 2. fru is necessary and sufficient for specifying male-specific vPN1 neurons.
(A, B) GFP expression (green) in the male (A) or female (B) brain of R72E10-GAL4AD/UAS-mCD8GFP; VT9665-GAL4DBD/+ flies counter-stained by nc82 antibody (magenta). vPN1 neurons with VLP projections were labeled in the male but not female brain. (C) vPN1 neurons (C1) in the male brain of R72E10-GAL4AD/UAS-mCD8GFP; VT9665-GAL4DBD/+ flies co-stained with FruM antibody (C2). (C3) is a merge of (C1) and (C2). (D) vPN1 neurons are present in the male brain (D1) but not female brain (D2) of fru4–40/+ flies. Genotype is: R72E10-GAL4AD/UAS-mCD8GFP; VT9665-GAL4DBD, fru4–40/+. (E) vPN1 neurons are absent in both the male brain (E1) and female brain (E2) of null mutant fru4–40/fruLexA. Genotype is: R72E10-GAL4AD/UAS-mCD8GFP; VT9665-GAL4DBD, fru4–40/fruLexA. (F) vPN1 neurons are present in both the male brain (F1) and female brain (F2) of fruM mutant flies where FruM is expressed in both males and females. Genotype is: R72E10-GAL4AD/UAS-mCD8GFP; VT9665-GAL4DBD, fruM/+.