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. 2015 Sep 21;4:e08477. doi: 10.7554/eLife.08477

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© 2015, Zhou et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — (A–C) Silencing aPN1 neurons decreased song-induced chaining responses. aPN1 drivers R21B12-GAL4 (A), R22B11-GAL4 (B), or R49F09-GAL4 (C) were crossed to UAS>stop>TNT; fru^LexA, LexAop2-FLP (TNT group), UAS>stop>TNTⁱⁿ; fru^LexA, LexAop2-FLP (TNTⁱⁿ group), or UAS>stop>TNT; LexAop2-FLP (no LexA group). (D, E) Silencing vPN1 neurons decreased song-induced chaining responses. vPN1 drivers R72E10-GAL4 (D), R46F09-GAL4 (E) were crossed to UAS>stop>TNT; fru^LexA, LexAop2-FLP (TNT group), UAS>stop>TNTⁱⁿ; fru^LexA, LexAop2-FLP (TNTⁱⁿ group), or UAS>stop>TNT; LexAop2-FLP (no LexA group). n = 13–16 for each condition. *p < 0.0001 compared to both controls at 80 dB, Wilcoxon rank-sum test. Shown in the left panel is GFP expression of each intersectional driver. In the right panel, a heat map summary shows chaining intensities across the testing time course. Each row corresponds to a group of six flies. Colors represent the number of flies in chain.

DOI: http://dx.doi.org/10.7554/eLife.08477.009