Figure 7. Optogenetic activation of auditory neurons.

(A–C) Optogenetic activation of vPN1 neurons (B) or pC1 neurons (C) induced male chaining, as evidenced by males courting each other on food (green arrow head), while activation of aPN1 neurons did not (A). CsChrimson activation was achieved with constant 655-nm light (0.06 mW/mm²). R22B11-GAL4 ∩ fru^LexA, R72E10-GAL4 ∩ fru^LexA, or R71G01-LexA ∩ dsx^GAL4 was used to drive CsChrimson expression in aPN1 (A), vPN1 (B), or pC1 (C). (D) Male-chaining behavior was induced by CsChrimson-mediated activation of vPN1 neurons but not aPN1 neurons. *p < 0.0001 (Student's t-test). n = 14–18 for all the genotypes. (E) Male-chaining behavior was induced by CsChrimson-mediated activation of pC1 neurons. *p < 0.0001 (Student's t-test). n = 16–18 for all the genotypes. (F, G) Male-chaining behavior induced by CsChrimson-mediated activation of either vPN1 or pC1 neurons when stimulated with constant red light at 0.006, 0.018, 0.03, 0.06 mW/mm² (F) or stimulated with 5-ms light pulses at 10, 25, 50, and 100 Hz (G). vPN1 driver 1 is R72E10-GAL4 ∩ fru^LexA; vPN1 driver 2 is R46F09-GAL4 ∩ fru^LexA, pC1 driver is R71G01-LexA ∩ dsx^GAL4. n = 16–28 for all the genotypes.

DOI: http://dx.doi.org/10.7554/eLife.08477.017