TABLE 2.
In vitro activities of four antifungal agents against nine clinical isolates of C. parapsilosis (sensu stricto) in the CLSI broth microdilution assay complemented by ERG11 sequence analysis and expression of the ERG11, CDR1, and MDR1 genes
| C. parapsilosis (sensu stricto) isolate | MIC (mg/liter) |
Mutations in ERG11 gene | Avg cDNA level (SD)a |
|||||
|---|---|---|---|---|---|---|---|---|
| FLC | VRC | ANF | AMB | ERG11 | CDR1 | MDR1 | ||
| LEMI 8646 | 8 | 1 | 2 | 0.25 | T591C, A395T | 7.4 (0.3) | 9.2 (0.6) | 1.5 (0.08) |
| LEMI 8650 | 8 | 0.5 | 2 | 0.25 | T591C, A395T | 3.7 (0.1) | 4.8 (0.1) | 0.9 (0.06) |
| LEMI 8653 | 16 | 0.5 | 2 | 0.25 | T591C, A395T | 1.5 (0.1) | 4.0 (0.3) | 0.9 (0.1) |
| LEMI 8655 | 16 | 0.5 | 1 | 0.25 | T591C, A395T | 3.3 (0.2) | 3.4 (0.1) | 0.8 (0.2) |
| LEMI 8657 | 16 | 0.5 | 2 | 0.125 | T591C, A395T | 3.9 (0.3) | 3.7 (0.2) | 1.0 (0.06) |
| LEMI 8662 | 16 | 0.5 | 2 | 0.25 | T591C, A395T | 5.7 (0.5) | 5.7 (0.5) | 1.3 (0.2) |
| LEMI 8379 | 8 | 0.5 | 2 | 0.25 | T591C, A395T | 4.0 (0.3) | 3.3 (0.3) | 0.8 (0.04) |
| LEMI 8382 | 8 | 0.5 | 2 | 0.25 | T591C, A395T | 7.3 (0.4) | 7.3 (0.5) | 1.2 (0.06) |
| LEMI 8383 | 8 | 0.5 | 1 | 0.25 | T591C, A395T | 4.4 (0.4) | 7.6 (0.6) | 1.0 (0.1) |
| ATCC 22019 | 0.5 | 0.5 | 1 | 0.125 | NDb | 1.0 (0.00) | 1.0 (0.07) | 1.0 (0.07) |
a
cDNA levels were calculated relative to average levels of cDNA obtained for the wild-type strain. The values are the averages from three replicates, and the standard deviations are in parentheses. The cDNA levels of the different genes were normalized to that of the ACT1 gene.
b
ND, none detected.