FIG 5.

GPL contents of M. abscessus cs1c-S cells with or without treatment with a subinhibitory dose of amikacin. (A) TLC analysis. Equal weights of total lipid extracts from M. abscessus cs1c-S cells with or without amikacin treatment were resolved by thin-layer chromatography with CHCl₃-CH₃OH-H₂O (100:14:0.8) and visualized with 1% 1-naphthol. (B) MALDI-TOF mass spectrometry of total lipids from M. abscessus cs1c-S cells with or without amikacin treatment and control strains 8858R (GPL-deficient rough type) and 8858S (GPL-abundant smooth type).