Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Kinetic constants of CTX-M-55 and CTX-M-15 toward cefotaxime at different temperatures
| CTX-M enzyme and kinetic constant | Value at a temp of: |
||
|---|---|---|---|
| 25°C | 40°C | 60°C | |
| CTX-M-55 | |||
| Km (μM) | 23 ± 3 | 45 ± 5 | 53 ± 6 |
| kcat (s−1) | 73 ± 4 | 95 ± 6 | 101 ± 6 |
| kcat/Km (μM−1 s−1) | 3.2 | 2.1 | 1.9 |
| CTX-M-15 | |||
| Km (μM) | 27 ± 2 | 56 ± 10 | 87 ± 12 |
| kcat (s−1) | 60 ± 2 | 32 ± 3 | 39 ± 4 |
| kcat/Km (μM−1 s−1) | 2.2 | 0.6 | 0.4 |
