Flow cytometry analysis of E. coli strains BW25113 (black bars and lines), JW0368 (orange bars and lines), and BS2 (blue bars and lines) incubated with Cf-labeled peptides (6 μM) for 90 min in 33% TSB medium. Geometric mean (GeoMean) fluorescence intensities and histogram plots are shown for cells treated with Cf-Api88 (A and B) and Cf-Onc112 (A and C). BW25113 treated without peptide as a control is shown as light gray area (B and C). Shown are the results of two independent experiments, as indicated by the error bars. **, P < 0.01 for JW0368 or BS2 versus BW25113. *, P < 0.05 for JW0368 or BS2 versus BW25113. #, P < 0.05 for BS2 versus JW0368.