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. 2015 Sep 18;59(10):5992–5998. doi: 10.1128/AAC.01307-15

TABLE 1.

E. coli strains and plasmids used in this study

Strain or plasmid Characteristics, sequence, or genotypea Reference or source
E. coli strains
    MC4100 F− araD139 Δ(argF-lac)205λ−rpsL150 flbB5301 relA deoC1 pstF25 44
    M1 to M24 MC4100 sbmA::Tn10 insertion mutants, Tetr This study
    BS1 to BS24 JW0368 yjiL::Tn10 insertion mutants, Tetr This study
    BS-L BW25113 ΔsbmA ΔyjiL Cmr This study
    BS-M BW25113 ΔsbmA ΔyjiM Cmr This study
    BS-N BW25113 ΔsbmA ΔyjiN Cmr This study
    BS-O BW25113 ΔsbmA ΔmdtM Cmr This study
    JW0368 BW25113 ΔsbmA Keio collectionb
    BW25113 lacIq rrnB3 ΔlacZ4787 hsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1 Keio collectionb
    JW5785 BW25113 ΔyjiL Keio collectionb
    JW4300 BW25113 ΔmdtM Keio collectionb
Plasmids
    psbmA pNTR-SD, sbmA Ampr Mobile plasmid collectionb
    pyjiL pNTR-SD, yjiL Ampr Mobile plasmid collectionb
    pKD46 Red recombinase expression plasmids, Ampr 37
    pKD3 Template plasmids for FRT-flanked chloramphenicol resistance cassette, Cmr 37
a

Tetr, tetracycline resistant; Cmr, chloramphenicol resistant; Ampr, ampicillin resistant.