FIG 1.

Effect of deferoxamine (DFM) on A. fumigatus biofilm formation and preformed A. fumigatus biofilm. (A) A. fumigatus conidia were exposed to DFM for 16 h, the disk was moved to new medium and incubated for an additional 24 h, and growth was assayed using XTT reduction, which was assessed by measuring the absorbance at 490 nm. (B) Preformed A. fumigatus biofilms were exposed to DFM for 24 h, and the resulting growth was assessed by XTT reduction by absorbance at 490 nm. Twelve-well assays were used. Each condition was performed in triplicate, and the results are pooled from two separate experiments on different days. Two asterisks indicate a significant P value (<0.01) compared to A. fumigatus control. (A) A DFM concentration of 2,500 μM enhanced A. fumigatus biofilm formation significantly more than 156 μM (P < 0.05), 312 μM (P < 0.05), and 625 μM (P < 0.01). (B) For preformed biofilms, 2,500 μM DFM was significantly more stimulatory than all other concentrations tested (P < 0.001, 156 μM; P < 0.01, 312 μM to 2,500 μM). The values shown are the means ± the standard deviations.