FIG 4.

Anti-T. cruzi immunoglobulin subclasses in serum of C57BL/6 mice infected with T. cruzi Y strain. The animals were treated with benznidazole (B) at 100, 50, 25, or 5 mg/kg alone or combined with suramin (S; 20 mg/kg) for a period of 15 days after confirmation of the infection (4 days after inoculation). INF, infected without treatment. Results for noninfected controls (NI) were suppressed in this figure since anti-T. cruzi immunoglobulins were not detected. Blood samples were collected 24 h after the last treatment. All animals that survived were investigated (NI, n = 10; INF, n = 6; B100, n = 10; S20, n = 4; B100 + S20, n = 10; B50 + S20, n = 8; B25 + S20, n = 6; B5 + S20, n = 5). Data are representative of the second experimental replicate from two independent studies with similar results. Different letters (a to f) denote a statistically significant difference among the groups (P < 0.05), and groups with a common letter are not statistically significantly different (P > 0.05).