TABLE 1.
Potency of GSK8853 against transient HCV replicons
| Genotype | Residue changea | Modified protein | EC50 (nM) | Fold shiftb | 95% confidence interval (nM) |
|---|---|---|---|---|---|
| 1a | Wild type | NS4B | 0.5 | 1.0 | 0.4–0.6 |
| F98L | NS4B | 25.8 | 51.6 | 22.3–30.6 | |
| V105L | NS4B | 3.7 | 7.4 | 2.5–6.0 | |
| V105M | NS4B | 173.2 | 346.4 | 124.9–243.0 | |
| 1a PBC002 | Wild type | NS4B | 1.6 | 1.0 | 1.3–2.0 |
| N56I | NS4B | >30,000 | >18,750 | ||
| N99H | NS4B | 7,364.5 | 4,602 | 5,224–10,505 | |
| 1b | Wild type | NS4B | 1.1 | 1.0 | 1.0–1.2 |
| H94N | NS4B | 37.7 | 34.3 | 34.7–41.0 | |
| H94Rc | NS4B | 52.6 | 47.8 | 41.9–66.0 | |
| F98Lc | NS4B | 27.1 | 24.6 | 23.6–31.2 | |
| V105Lc | NS4B | 6.6 | 6.0 | 6.0–7.2 | |
| V105M | NS4B | 179.0 | 162.7 | 159.4–200.9 | |
| R155Q | NS3 | 2.6 | 2.4 | 2.3–3.4 | |
| A156Tc | NS3 | 0.9 | 0.8 | 0.60–1.5 | |
| D168A | NS3 | 1.8 | 1.6 | 1.1–7.9 | |
| L28V | NS5A | 1.6 | 1.5 | 1.5–1.8 | |
| L31Vc | NS5A | 1.4 | 1.3 | 1.0–3.3 | |
| Y93H | NS5A | 1.1 | 1.0 | 0.7–1.9 | |
| C316Y | NS5B | 1.5 | 1.4 | 1.1–3.0 | |
| M414Tc | NS5B | 1.2 | 1.1 | 0.6–8.2 | |
| P495L | NS5B | 1.1 | 1.0 | 0.9–2.0 |
a
Variants are numbered according to the translated peptide sequence of the gene in which the mutation is located.
b
Fold shift is calculated for mutant replicons relative to the unaltered (wild-type) clone of the matching background.
c
Data were originally reported in reference 7.