TABLE 2.
Annotated differentially expressed proteins in C. albicans biofilm persisters
| Proteins by function | UniProt accession no. | Gene | Log2 fold change | P value |
|---|---|---|---|---|
| Glycolysis | ||||
| Fructose-bisphosphate aldolase | Q9URB4 | FBA1 | −2.83 | 3.96E-09 |
| Enolase 1 | P30575 | ENO1 | −2.02 | 3.64E-07 |
| Hexokinase-2 | P83776 | HXK2 | −3.84 | 1.53E-13 |
| Phosphoglycerate kinase | P46273 | PGK1 | −3.42 | 1.11E-17 |
| Phosphoglycerate mutase | P82612 | GPM1 | −0.67 | 0.0013 |
| Triosephosphate isomerase | Q9P940 | TPI1 | −2.63 | 3.02E-12 |
| Hexokinase-like proteina | Q59K93 | GLK2 | −0.84 | 5.13E-07 |
| Likely hexokinasea | Q59TZ8 | GLK1 | ||
| Likely hexokinasea | Q59JX3 | GLK3 | ||
| Likely hexokinasea | Q59RR7 | GLK4 | ||
| Uncharacterized protein | Q5AK23 | ADH1 | −1.39 | 3.24E-08 |
| Pentose phosphate pathway | ||||
| Potential 6-phosphogluconolactonase | Q59PZ6 | SOL3 | −3.53 | 3.21E-07 |
| Transaldolase | Q5A017 | TAL1 | −1.24 | 2.96E-09 |
| 6-Phosphogluconate dehydrogenase, decarboxylating | Q5AKV3 | GND1 | −0.64 | 3.46E-05 |
| Glucose-6-phosphate 1-dehydrogenasea | Q5APL0 | ZWF1 | −1.41 | 2.97E-14 |
| Glucose-6-phosphate 1-dehydrogenasea | Q5AQ54 | |||
| Gluconeogenesis | ||||
| Uncharacterized protein | Q5AAH5 | PCK1 | 0.97 | 0.0006 |
| Uncharacterized protein | G1UB66 | FBP1 | 0.71 | 0.0064 |
| TCA cycle | ||||
| Aconitate hydratase, mitochondrial | P82611 | ACO1 | −1.12 | 1.58E-07 |
| Malate dehydrogenase, cytoplasmic | P83778 | MDH1 | −0.60 | 0.0006 |
| Malate dehydrogenase | Q5AMP4 | MDH1-1 | −0.92 | 1.28E-06 |
| Isocitrate dehydrogenase (NADP) | Q59MF7 | IDP1 | −1.75 | 4.22E-11 |
| Isocitrate dehydrogenase (NADP) | Q59V07 | IDP2 | −1.43 | 2.73E-07 |
| Putative uncharacterized protein IDH2a | Q5A0M1 | IDH2 | 2.70 | 2.46E-07 |
| Putative uncharacterized protein IDH2a | Q5A0T8 | |||
| Citrate synthase | Q59ZZ5 | CIT1 | 2.61 | 4.93E-12 |
| Glyoxylate cycle | ||||
| Isocitrate lyase | Q59RB8 | ICL1 | 3.87 | 1.75E-08 |
| Malate synthase | Q5APD2 | MLS1 | 2.73 | 2.61E-13 |
| Biosynthesis of amino acids | ||||
| Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine inhibited | P79023 | ARO4 | −2.32 | 2.44E-09 |
| 5-Methyltetrahydropteroyltriglutamate-homocysteine methyltransferase | P82610 | MET6 | −2.17 | 7.23E-11 |
| Uncharacterized protein | Q5A9D9 | LYS12 | −2.48 | 1.17E-13 |
| Homoserine dehydrogenase | Q5AIA2 | HOM6 | −2.99 | 3.17E-08 |
| Uncharacterized protein | Q5ALQ9 | ARO3 | −0.74 | 0.0018 |
| Phosphoserine aminotransferase | Q59P52 | SER1 | −2.29 | 2.97E-07 |
| Branched-chain amino acid aminotransferase | Q59YS9 | BAT22 | −2.87 | 1.80E-13 |
| Branched-chain amino acid aminotransferase | Q5AHJ9 | BAT21 | −2.44 | 1.79E-08 |
| Uncharacterized protein | Q5AAM0 | ARG1 | −0.98 | 0.0004 |
| 3-Isopropylmalate dehydrogenase | Q5AFI8 | LEU2 | −2.33 | 6.64E-12 |
| Likely mitochondrial ketol-acid reductoisomerasea | Q59WW5 | ILV5 | −1.59 | 6.45E-10 |
| Likely mitochondrial ketol-acid reductoisomerasea | Q59XR8 | |||
| Asparagine synthetasea | Q59SH9 | ASN1 | −3.05 | 2.09E-10 |
| Asparagine synthetasea | Q59SE8 | |||
| Translational elongation factor | ||||
| EF-1α1a | P0CY35 | TEF1 | −0.80 | 6.06E-06 |
| EF-1αa | Q59K68 | |||
| EF-1α2a | Q59QD6 | |||
| EF-2 | Q5A0M4 | EFT2 | −1.55 | 1.61E-08 |
| EF-3 | P25997 | CEF3 | 2.34 | 3.09E-09 |
| EF-Tu | Q5ABC3 | TUF1 | −1.09 | 0.0005 |
| Potential translation elongation factor Cam1p | Q59QS2 | CAM1-1 | 2.06 | 1.88E-07 |
| Putative uncharacterized protein EFB1 | Q5A652 | EFB1 | 4.29 | 7.72E-09 |
| Regulation of Ran GTPase activity | ||||
| Uncharacterized proteina | Q5AJS1 | RNA1 | −3.11 | 4.46E-12 |
| Uncharacterized proteina | Q5AJE2 | |||
| Ribosome | ||||
| Likely cytosolic ribosomal protein S3 | Q59N00 | RPS3 | 3.34 | 9.66E-11 |
| Ubiquitin-ribosomal protein fusion S27aa | Q5A109 | UBI3 | −0.81 | 6.65E-05 |
| Uncharacterized proteina | Q5ADS0 | UBI4 | ||
| Likely cytosolic ribosomal acidic protein P0 | Q5AFQ4 | RPP0 | 3.60 | 1.26E-10 |
| Likely cytosolic ribosomal protein S18 | Q5AFQ0 | RPS18 | 1.74 | 1.04E-05 |
| Likely cytosolic ribosomal protein S20 | Q5A389 | RPS20 | 3.30 | 6.08E-08 |
| Likely cytosolic ribosomal protein L12 | Q5AJF7 | RPL12 | 2.30 | 0.0003 |
| Likely cytosolic ribosomal protein S5 | Q5AG43 | RPS5 | 3.60 | 3.38E-10 |
| Likely cytosolic ribosomal protein S7 | Q5AJ93 | RPS7A | 3.32 | 2.42E-14 |
| Likely cytosolic ribosomal protein L4 | Q59ZX4 | RPL4B | 1.58 | 4.61E-08 |
| 40S ribosomal protein S0 | O42817 | RPS0 | 2.41 | 1.86E-09 |
| 40S ribosomal protein S1 | P40910 | RPS1 | 2.72 | 8.23E-10 |
| 40S ribosomal protein S6 | Q5AMI6 | RPS6A | 3.91 | 4.81E-08 |
| 40S ribosomal protein S12 | Q5ADQ6 | RPS12 | 2.40 | 1.13E-06 |
| Heat shock proteins | ||||
| HSP90 homolog | P46598 | HSP90 | 3.09 | 2.26E-13 |
| Small HSP21 | Q5AHH4 | HSP21 | 3.85 | 1.86E-10 |
| Putative uncharacterized protein HSP104 | Q5A376 | HSP104 | 3.56 | 3.55E-06 |
| HSP SSA1 | P41797 | SSA1 | 0.36 | 0.0004 |
| HSP SSC1, mitochondrial | P83784 | SSC1 | 0.96 | 2.23E-07 |
| Likely HSP70/BiP chaperonea | Q5AD54 | KAR2 | 2.25 | 6.29E-06 |
| Uncharacterized proteina | Q5ADI3 | |||
| Response to abiotic stimulus | ||||
| 14-3-3 protein homolog | O42766 | BMH1 | 2.58 | 1.46E-10 |
| Ras-like protein 1 | Q59XU5 | RAS1 | 1.22 | 0.0004 |
| Guanine nucleotide-binding protein subunit β-like protein | P83774 | ASC1 | 1.79 | 7.89E-08 |
| Uncharacterized protein | Q59W54 | SMT3 | 1.53 | 0.0003 |
| Putative uncharacterized protein | Q5ADT0 | SLK19 | 3.27 | 1.25E-12 |
| Adhesion | ||||
| Agglutinin-like protein 3 | Q59L12 | ALS3 | 2.45 | 0.0002 |
| Cell surface mannoprotein MP65 | Q59XX2 | MP65 | 2.41 | 6.67E-08 |
| Cell surface protein | Q5A9D4 | CSP37 | 3.19 | 8.36E-11 |
| Ergosterol biosynthesis | ||||
| NADH-cytochrome b5 reductase 2 | Q59M70 | MCR1 | −1.79 | 8.68E-09 |
| Uncharacterized protein | Q59K50 | ERG10 | −0.75 | 0.001 |
| Uncharacterized protein | Q5A3Z7 | ERG13 | −1.64 | 2.96E-07 |
| Cell wall integrity | ||||
| Glucan 1,3-β-glucosidase | P29717 | XOG1 | 1.32 | 0.0013 |
| Glucan 1,3-β-glucosidase BGL2 | Q5AMT2 | BGL2 | 2.44 | 2.83E-09 |
| Secreted β-glucosidase SUN41 | Q59NP5 | SUN41 | 3.27 | 1.24E-06 |
| Secreted β-glucosidase SIM1 | Q5AKU5 | SIM1 | 3.51 | 1.53E-09 |
| Uncharacterized protein | Q5AAH5 | PCK1 | 0.97 | 0.0006 |
| pH-responsive protein 2 | O13318 | PHR2 | 0.64 | 0.0001 |
| Mannose-1-phosphate guanyltransferase | O93827 | MPG1 | 1.52 | 3.83E-05 |
| Potential cell wall glucanase | Q5AKC7 | SCW11 | 3.51 | 4.75E-09 |
| Uncharacterized protein | Q5AL34 | PSA2 | 0.64 | 0.0061 |
| Possible sphingolipid long-chain-base sensory protein | Q59KV8 | LSP1 | 3.60 | 2.02E-14 |
| Possible sphingolipid long-chain-base sensory protein | Q59VF6 | PIL1 | 3.22 | 2.22E-16 |
| Response to oxidative stress | ||||
| Putative NADPH-dependent methylglyoxal reductase GRP2 | P83775 | GRP2 | 2.41 | 5.27E-11 |
| Peroxiredoxin TSA1 | Q9Y7F0 | TSA1 | 2.95 | 1.44E-12 |
| Potential mitochondrial glutaredoxin | Q5ABB1 | TTR1 | 1.50 | 0.0007 |
a
Protein group.