FIG 1.

Antiproliferative effects of E5700, ITZ, and POSA, alone and in combination, against L. amazonensis promastigotes. Parasites were treated with different concentrations for 72 h to evaluate growth. Several combinations between E5700, ITZ, and POSA were tested. The arrows indicate the time of addition of the drugs at the indicated concentrations. The MIC values represent the MIC that produced total death of the parasite population in culture. Isobolograms illustrate the combined effects between E5700 and ITZ or POSA on L. amazonensis promastigotes. The FIC was calculated according to the method described by Hallander et al. (27). FIC values less than 0.5 indicate a synergistic effect.