FIG 7.

Ultrathin sections of L. amazonensis intracellular amastigotes. (A) Control intracellular amastigotes. (B to D) Amastigotes treated with different combinations of E5700 and ITZ or POSA for 48 h: 1.25 nM E5700 plus 40 nM ITZ (B and C); 0.625 nM E5700 plus 5 nM POSA (D). Different ultrastructural alterations were observed: (i) presence of some vacuoles containing many small vesicles and membrane profiles similar to autophagosomes, sometimes close to organelles such as the mitochondrion and nucleus (B to D, arrows); (ii) mitochondrial swelling and appearance of circular cristae (C); (iii) presence of lipid bodies (C and D; asterisks). N, nucleus; m, mitochondrion; k, kinetoplast; f, flagellum; A, autophagosome.