TABLE 2.
Cytotoxicity of divalent metal ion saltsa
| Culture medium or salt (1 μM) | Relative viability (%) |
|
|---|---|---|
| Mean ± SE | P | |
| Culture medium | 100 | |
| CuSO4 | 85.7 ± 2.7 | * |
| ZnCl2 | 80.8 ± 2.9 | ** |
| ZnSO4 | 81.0 ± 4.6 | ** |
| AgNO3 | 93.9 ± 2.3 | NS |
| MnCl2 | 97.8 ± 4.7 | NS |
| MgCl2 | 74.1 ± 5.0 | *** |
| MgSO4 | 85.2 ± 3.6 | * |
| NiSO4 | 77.7 ± 1.5 | *** |
| LiCl | 77.1 ± 3.6 | *** |
a
The HepG2 survival for each divalent metal ion salt was determined using culture medium as a reference of 100% viability; the average luminescence of each salt was used to calculate the percent viability compared to the control. The data represent the means from three different experiments. Statistical differences were calculated using the Mann-Whiney test (GraphPad, Prism 5.0 software). *, P < 0.05; **, P < 0.01; ***, P < 0.001; NS, not significant.