TABLE 2.
Calculated and detected RRTs for sterol TMS ethers
| Sterol | Calculated RRT | Detected RRT |
|---|---|---|
| 3β,6α-Diol sterols | ||
| 14-Methylergosta-8,24(28)-dien-3β,6α-diol (compound 4) | 1.44 | 1.43 |
| 14-Methylergosta-7,24(28)-dien-3β,6α-diol (compound 5) | 1.47 | 1.49 |
| 14-Methylergosta-8,22,24(28)-trien-3β,6α-diol (compound 6) | 1.41 | 1.39 |
| 14-Methylergosta-8,22-dien-3β,6α-diol (compound 7) | 1.36 | 1.36 |
| 14-Methylergosta-8-en-3β,6α-diol (compound 8)a | 1.40 | |
| 14-Methylergosta-7,22,24(28)-trien-3β,6α-diol (compound 9)a | 1.44 | |
| 14-Methylergosta-7-en-3β,6α-diol (compound 10)a | 1.44 | |
| 14-Methylergosta-7,22-dien-3β,6α-diol (compound 11)a | 1.40 | |
| 4,4,14-Trimethyl sterols | ||
| 4,4,14-Trimethylergosta-8,24(28)-dien-3β-ol (eburicol) (compound 2) | 1.51 | 1.51 |
| 4,4,14-Trimethylergosta-8,22,24(28)-trien-3β-ol (compound 14)a | 1.48 | |
| 4,4,14-Trimethylergosta-8,22-dien-3β-ol (compound 15)a | 1.45 | |
| 4,4,14-Trimethylergosta-7,22-dien-3β-ol (compound 16) | 1.49 | 1.48 |
| 4,4,14-Trimethylergosta-8-en-3β-ol (compound 17)a | 1.48 | |
| 4,4,14-Trimethylergosta-7,24(28)-dien-3β-ol (compound 18)a | 1.55 |
a
This sterol has not been detected in our investigation but is postulated on the basis of expected biochemical conversions.