TABLE 1.
Overview of primers used in this study
| Primer | Gene product (accession no.a) | Sequence |
|---|---|---|
| NcSAG1-F | N. caninum SAG1 (FR823385; region 1666638–1673366) | GGGTCGTAGGCTTGATGG |
| NcSAG1-R | GCAGCAGCGGCAAGGGG | |
| TgSAG1-F | T. gondii SAG1 (TGME49_264660) | CTTCAGGACGTCGTTCCC |
| TgSAG1-R | CACCTTTGCAGGCTTGCC | |
| NcBAG1_F | N. caninum BAG1 (NCLIV_chrVIIb; region 2868187–2869925) | CTCGACTTCATGGATGAGG |
| NcBAG1_R | CTTCTATGGTAACGTCATCC | |
| TgBAG1-F | T. gondii BAG1 (TGME49_259020) | CTAGACTATTTGGAT |
| TgBAG1-R | CTGTCGAACTCCACG | |
| NcGra2_F | N. caninum GRA2 (AF196293.1) | GCCATGCGTGTATCGCCC |
| NcGra2_R | TTCGCAGTAAACTGCTTGAG | |
| TgGra2_F | T. gondii GRA2 (TGME49_227620) | CCAGAAGAACCGGTTTCCC |
| TgGra2_R | AACGGTCACCATGCCCCT |
a
The accession numbers for T. gondii SAG1, BAG1, and GRA2 are from ToxoDB (http://www.toxodb.org), and the accession number for N. caninum BAG1 is from GeneDB (http://www.genedb.org).