TABLE 5.

Effects of BKI-1294 treatments on clinical signs, mortality, fertility, and cerebral N. caninum Nc-Spain7 infection in nonpregnant mice, dams, and pups^a

Parameter	BKI-1294	Placebo control
Nonpregnant females (no./total no.)
Total no.	6	8
Clinical signs	0/6	0/8
Mortality	0/6	0/8
Seropositive	6/6	8/8
N. caninum positive	1/6b	8/8
Dams and pups (no./total no.)
Total no. of dams	10	8
Clinical signs	2/10c	5/8
Mortality	0/10d	1/8
Seropositive dams	10/10	8/8
N. caninum-positive dams	3/10b	8/8
Pregnancy rate	10/16	8/16
Total no. of pups	58	43
Litter size avg (no. of pups/no. of dams)	58/10d	43/8
Neonatal mortalitye	2/58d	2/43
Postnatal mortalityf	4/56g	40/41
N. caninum-positive pups	9/58g	43/43

^aMice were treated with BKI-1294 in corn oil or with corn oil alone as a placebo control, infected with Nc-Spain7, and sacrified as described in Materials and Methods. Adults and surviving pups were tested for the presence of N. caninum in their brains with a specific real-time PCR. Pups that had died before the end of the experiment were considered N. caninum positive. Respective numbers of animals in control and 1294-treated groups were compared by chi-square tests.

^bP < 0.005.

^cP < 0.1.

^dP > 0.1.

^eProportion of pups born dead or that died within the 2 first days postpartum.

^fProportion of pups dead from days 3 to 30 postpartum.

^gP < 0.001.