TABLE 1.

Primers used in this study

Primer name	Primer sequence (5′ to 3′)
Construction of cassettes
MRR1_F1	GGGGGTACCCTACTGATATGCCTGACGCCAC
MRR1_R1	GGGGGGCCCTCTCTCTTATTGAAAACAAGAAAGC
MRR1_F2	TCCCCGCGGCTACTGATATGCCTGAGGCCAC
MRR1_R2	GGGGAGCTCTCTCTCTTATTGAAAACAAGAAAGC
Site-directed mutagenesis
MRR1VSDM_F1	CGAGGTATTTTTACGCATGGAAATTGACAAAGAGTCATTCTTATT
MRR1VSDM_R1	AATAAGAATGACTCTTTGTCAATTTCCATGCGTAAAAATACCTCG
MRR1VSDM_F2	GCGGCCCCAGCAACAACAGCCTATAGGG
MRR1VSDM_R2	CCCTATAGGCTGTTGTTGCTGGGGCCGC
PCR confirmation
MRR1_F3	GAAAACAAGTAATCAAAACACGGGG
FLP_R	TTTATGATGGAATGAATGGGATG
MRR1_F4	CGGCATCTCGCAGCAACAA
MRR1_R3	GTCTGTAAAGGGGGGGGTTGGA
MRR1_R4	ACTTGAACGAAATGGAGACC
qRT-PCR
TUB4_F	TGTATTCCACAATGATGCCT
TUB4_R	TGCCTTGAAACGAAGTAGC
MRR1_F	ACAATGGTCTGAGCAATGAA
MRR1_R	GGCAATACTGGTGATGGAA
MDR1_F	TTCGTGATAGTTTTGGTGGTAG
MDR1_R	TGAACCTGGAGTGAATCTTGT