(A and B) Bactericidal activities of CSA-13 (○), CSA-13–IRDye 800CW (●), and IRDye 800CW (□) against planktonic (A) and biofilm-forming (B) P. aeruginosa PAO1. Results represent means ± SDs from three experiments. (C and D) CSA-13–IRDye 800CW activities in abdominal fluid (C) and in vivo in mice that received CSA-13–IRDye 800CW at a concentration of 5 μg/g (D). Results represent means ± SDs (n = 5). *, statistically significant (P ≤ 0.05), compared to untreated control. (E) Total amounts of WBCs in the blood of healthy mice (control), infected mice (untreated), and infected mice treated with CSA-13–IRDye 800CW after PAO1 challenge. Results represent means ± SDs (n = 5). (F) Square root of the spleen weight/total body weight ratio for healthy, CSA-13–IRDye 800CW-treated, and untreated mice 8 h after PAO1 injection. Results represent means ± SDs (n = 5).