FIG 2.

Antibiotic concentrations present in the dialysates. Antibiotic concentrations were measured in dialysates from samples of 400 μg of each antibiotic (in 1 ml PBS) that were placed into dialysis tubing with (+) or without (−) 125 mg porcine gastric mucin at 4°C, except for the top right panel, for which data were obtained at 37°C. The percentage of the antibiotic present in each dialysate was calculated relative to the theoretical concentration based on the total volume of the solution. Mean and standard deviation (SD) values are plotted for assays performed multiple times. Colistin sulfate dialysis at 4°C was performed in triplicate; dialysis of ciprofloxacin and daptomycin was performed once, and the others were performed twice. Error bars for colistin sulfate at 37°C and tobramycin were too small to show.