Author response image 1.
Dye saturation does not occur under our experimental conditions. Top. Summary of peak ∆G/R from dendritic shaft in response to different stimulation patterns. Only the experiments in which TBS of the PP paired with somatic current injection to evoke bAPs are shown here to demonstrate that the largest peak ΔG/R achievable by physiological activities in our settings was above the largest ΔG/R in response to single high‐frequency burst stimulation in the dataset (Figure 7 and Figure 8–figure supplement 1). A total of 15 different stimulation conditions (i.e., combinations of different calcium concentrations in the ACSF, synaptic stimuli and somatic current injections) were tested. Bottom. Representative traces of ∆G/R from dendritic shaft and voltage from somatic current-clamp recording in response to TBS paired or not paired with somatic current injection. Also note that the dye was not saturated by a single burst in general because ΔG/R was able to facilitate upon delivery of additional bursts in TBS with the ratio ΔG/R(Max):ΔG/R(1st burst) = 1.52 ± 0.31 (n = 4 cells, in 2 mM Ca2+).