Figure 1.
Generation of H7N9 avian influenza virus-like particles. (A) Analysis of virus-like particles (VLPs) in culture supernatants by Western blotting using H7N9 infected patient serum (lane 1) and mouse serum immunized by H7N9 virions (lane 2) to identify the expression of particles. Three expected bands with the molecular weight of 75 kD, 68 kD, and 28 kD, are equal to the size of hemagglutinin (HA), neuraminidase (NA), and matrix 1 (M1) of VLPs; (B) Identification of H7N9 avian influenza VLPs by transmission electron microscopy. Transfected 293T cells were fixed, dehydrated, embedded, stained with lead citrate and uranyl acetate, and observed by electron microscopy.